What must be documented on a license regarding blood collection?

The correct answer highlights the importance of documenting the volume and frequency of blood collection for several reasons. This information is critical for maintaining animal welfare standards and ensuring that the procedures adhere to legal and ethical guidelines. Documentation of volume helps to assess whether the amount of blood taken is within safe limits for the specific species being used, which varies widely among different animals. Additionally, recording the frequency of blood collection allows for monitoring to prevent over-collection, which could lead to adverse effects on the health and well-being of the animals. This level of oversight is essential for research protocols to ensure compliance with regulations and ethical standards in animal care.

The other options, while they may involve relevant aspects of the blood collection process, do not capture the primary regulatory focus of volume and frequency, which directly impacts the welfare of the animal and the integrity of the research data collected. For instance, while knowing the type of animal and reason for collection is crucial for context and justification, it does not address the specific procedural details that ensure animal safety during the blood collection process. Similarly, the documentation of collection location and staff involved may be important for operational reasons but does not directly pertain to the health implications of how much blood is collected or how often. Lastly, the duration of the collection