What characterizes a 'sub-threshold' procedure in animal research?

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A 'sub-threshold' procedure in animal research is characterized by being an authorized procedure that is designed to cause no pain or distress to the animals involved. The term 'sub-threshold' refers specifically to interventions or activities that remain below the threshold of causing any significant adverse effects on the animals' well-being.

This implies that such procedures are carefully regulated and executed to ensure the welfare of the animals and comply with ethical standards in animal research. By maintaining this level of care, researchers can achieve their scientific objectives while minimizing any potential harm to the animals. This focus on preventing pain and distress is a core principle of responsible animal research and aligns with the ethical considerations mandated by various regulations and guidelines governing such studies.

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